Protein_Domain
Part:BBa_K1781002:Design
Designed by: Marvin Prein Group: iGEM15_TU_Dresden (2015-09-09)
ZHER2 - affibody with affinity to cytoplasmic domain of HER2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 220 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
As it is only 58 amino acids long, the small size of the sequence can be problematic towards cloning and recombineering procedures
Source
The sequence was received from the scientific paper by Charles Eigenbrot in 2010, who engineered ZHER2 to have a better affinity towards HER2.